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- 品牌：Polyplus
- 產(chǎn)地：中國
- 型號：0.4 mL
- 貨號：101000010
- 價(jià)格： ￥20/盒
- 發(fā)布日期： 2024-03-05
- 更新日期： 2024-08-12
| 產(chǎn)地 | 中國 |
| 保存條件 | -20℃ |
| 品牌 | Polyplus |
| 貨號 | 101000010 |
| 用途 | 詳詢 |
| 組織來源 | 詳詢 |
| 細(xì)胞形態(tài) | 詳詢 |
| 是否是腫瘤細(xì)胞 | 否 |
| 保質(zhì)期 | 一年 |
| 器官來源 | 詳詢 |
| 免疫類型 | 詳詢 |
| 品系 | 詳詢 |
| 生長狀態(tài) | 詳詢 |
| 物種來源 | 詳詢 |
| 包裝規(guī)格 | 0.4 mL |
| 是否進(jìn)口 | 否 |
Specifications
| Reagent |
PULSin® |
|---|---|
| Molecule delivered |
Protein; Antibody; Peptide |
| Cell types |
Adherent & suspension cells grown in presence of serum |
| Number of transfection |
0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates |
| Storage |
Store PULSin® at 5 °C ± 3°C. Do not freeze. |
| Provided with |
Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control |
Overview
The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.
PULSin®?is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.
Ordering Information
| Reference number | Amount of reagent | Positive control (R-phycoerythrin) | Hepes dilution buffer |
|---|---|---|---|
| 101000010 | PULSin® 0.4 mL | 20 μg | 20 ml |
