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Product Introduction:

NHS (N-hydroxysuccinimide)-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

NHS-activated SepFast media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.

Product Properties:

NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with N-hydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFast media is supplied as a suspension in isopropanol.

Product Series: