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Product Introduction:

Irreversible Thiol-coupling SepFast MAG is used to make magnetic adsorbents to immobilize thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or special equipment is required.

Irreversible Thiol-coupling SepFast MAG medium forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm (12 atoms) that facilitates much improved ligand accessibility. This pre-activated magnetic agarose resin can be readily employed to make various magnetic affinity chromatography media for both small scale and large scale purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.

Product Properties:

There is a choice of two different activated base matrices that suit ligands of various molecular weights.

• Irreversible Thiol-Coupling SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions.

• Irreversible Thiol-Coupling SepFast MAG 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity and allows harsher operational conditions.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated by attaching a haloacetyl group through a long hydrophilic spacer arm (12 atoms). It reacts rapidly with molecules containing thiol groups. Irreversible Thiol-coupling SepFast media is supplied as an aqueous suspension.

Product Series: