肼活化磁性瓊脂糖層析填料

Product Introduction:

Hydrazide activated SepFast MAG is a magnetic agarose resin that is particularly useful for making glycoprotein-attached magnetic adsorbents. 

Hydrazide-activated agarose permits the coupling of aldehyde- or ketone-containing ligands through the formation of stable hydrazone linkages. It is a particularly powerful way to immobilize glycoproteins via their carbohydrate chains while leaving critical active sites intact. It is a method called “site-directed immobilization”. For example, antibodies can be immobilized to an agarose solid support through a linkage to their glycosylated side chain that leaves their Fab region free for antigen binding. 

Glycoproteins can be readily oxidized with sodium periodate to generate formyl groups on their carbohydrate chains before the coupling reaction with Hydrazide-activated SepFast MAG. It is a successful and well-documented technique. The coupling reaction is mild and easy to carry out. No toxic chemical or special equipment is required. 

Hydrazide-activated SepFast MAG resins can be readily employed to make custom affinity chromatography media for both small scale and large scale purification applications.

Product Properties:

Hydrazide activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and high chemical stability for harsher operational conditions. 

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. 

The base matrix is activated with hydrazide through a long hydrophilic spacer arm. Hydrazideactivated SepFast MAG media is supplied as an aqueous suspension.

Group to be coupled：-HC=O (aldehyde), -C=O (ketone)

Matrix  SepFast MAG 4HF: highly cross-linked 4% magnetic agarose

Particle size*：50 – 150 μm

Coupling level：>2 mg hIgG / ml medium

pH stability：4 -11 (ligand dependent)

Chemical stability：Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can with stand 

Storage：+4℃ - +8℃

Product Series: