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- 品牌：Invivogen
- 產(chǎn)地：國外
- 型號：詳詢
- 貨號：bb-kostg
- 發(fā)布日期： 2023-03-31
- 更新日期： 2024-08-12
| 產(chǎn)地 | 國外 |
| 保存條件 | 詳詢 |
| 品牌 | Invivogen |
| 貨號 | bb-kostg |
| 用途 | 詳詢 |
| 組織來源 | 詳詢 |
| 細胞形態(tài) | 詳詢 |
| 是否是腫瘤細胞 | 是 |
| 保質(zhì)期 | 詳詢 |
| 器官來源 | 詳詢 |
| 免疫類型 | 詳詢 |
| 品系 | 詳詢 |
| 生長狀態(tài) | 詳詢 |
| 物種來源 | 詳詢 |
| 包裝規(guī)格 | 詳詢 |
| 是否進口 | 是 |
B16-Blue™ ISG-KO-STING cells can be used for the study of the STING signaling pathway and for the detection of bioactive murine types I and II IFNs by monitoring the activation of the JAK/STAT pathway.
B16-Blue™ ISG-KO-STING cells were generated from the B16-Blue™ ISG cell line, a murine B16- F1 melanoma-derived cell line, through stable knockout of the STING gene. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
B16-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, DMXAA, canonical and non-canonical CDNs while retaining the ability to respond to type I and type II IFNs.
Stimulation of these cells with IFN triggers the activation of the I-ISG54 promoter and the production of SEAP. Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.
