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- 品牌：Invivogen
- 產地：國外
- 型號：詳詢
- 貨號：bb-ifng
- 發(fā)布日期： 2023-03-30
- 更新日期： 2024-08-12
| 產地 | 國外 |
| 保存條件 | -20℃保存 |
| 品牌 | Invivogen |
| 貨號 | bb-ifng |
| 用途 | 科研 |
| 組織來源 | 詳詢 |
| 細胞形態(tài) | 詳詢 |
| 是否是腫瘤細胞 | 是 |
| 保質期 | 詳詢 |
| 器官來源 | 詳詢 |
| 免疫類型 | 詳詢 |
| 品系 | 詳詢 |
| 生長狀態(tài) | 詳詢 |
| 物種來源 | 詳詢 |
| 包裝規(guī)格 | 詳詢 |
| 是否進口 | 是 |
Cell line description:
B16-Blue™ IFN-γ cells derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP (secreted embryonic alkaline phosphatase) reporter gene under the control of the IFN-inducible ISG54 promoter enhanced by a multimeric interferon-sensitive response element (ISRE).
B16-Blue™ IFN-γ cells do not respond to IFN-α/β, due to the inactivation of the type I IFN receptor. B16-Blue™ IFN-γ cells respond specifically to mIFN-γ and do not respond to human IFN-γ.
Stimulation of B16-Blue™ IFN-γ cells with mIFN-γ triggers the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™ Solution, a medium that turns purple/blue in the presence of SEAP and by reading the OD at 655 nm.
