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- 品牌：InVivoGen
- 產(chǎn)地：國(guó)外
- 型號(hào)：詳詢
- 貨號(hào)：a549d-nfis
- 發(fā)布日期： 2023-03-28
- 更新日期： 2024-08-12
| 產(chǎn)地 | 國(guó)外 |
| 保存條件 | -20℃保存 |
| 品牌 | InVivoGen |
| 貨號(hào) | a549d-nfis |
| 用途 | 科研 |
| 組織來源 | 詳詢 |
| 細(xì)胞形態(tài) | 詳詢 |
| 是否是腫瘤細(xì)胞 | 是 |
| 保質(zhì)期 | 詳詢 |
| 器官來源 | 詳詢 |
| 免疫類型 | 詳詢 |
| 品系 | 詳詢 |
| 生長(zhǎng)狀態(tài) | 詳詢 |
| 物種來源 | 詳詢 |
| 包裝規(guī)格 | 詳詢 |
| 是否進(jìn)口 | 是 |
Human NF-κB-SEAP & IRF-Luc Reporter lung carcinoma
A549-Dual™ cells are adherent epithelial cells that have been derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs. The A549 cell line is a well-characterized cellular model for asthma, allergies and respiratory infections.
A549-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites.
A549-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.
As a result, A549-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase.
Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
