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Product Introduction:

Aldehyde-activated SepFast MAG is a magnetic resin for the preparation of custom magnetic affinity chromatography media. The magnetic particles can be readily controlled and isolated in biological feedstock with the aid of a magnet. Coupling biospecific ligands to aldehyde-activated agarose is a successful and well-documented technique.

Aldehyde-activated SepFast MAG forms chemically stable secondary amine linkages with ligands containing primary amine groups through reductive amination reactions. This pre-activated agarose base matrix can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications.

Product Properties:

There is a choice of 2 different base matrices that suit various molecules to be coupled or purified.

• Aldehyde-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. Its large pore size is suitable for coupling or purifying large molecules. It shows high mechanical rigidity allowing harsher operational conditions.

• Aldehyde-activated SepFast 6HF is made of highly cross-linked 6% agarose containing magnetic material. Its moderate pore size is suitable for coupling smaller molecules. It shows high mechanical rigidity allowing harsher operational conditions.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated through oxidation to generate aldehyde groups. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of reducing agents. Aldehyde-activated SepFast MAG media is supplied as an aqueous suspension in 20% ethanol.