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Product Introduction:

Glutathione SepFast MAG is a magnetic affinity chromatography medium used for the rapid one-step purification of Glutathione S-transferase (GST)-tagged proteins with the aid of a magnet. Other Glutathione S-transferases and Glutathione-binding proteins can also be purified with this adsorbent. 

Reduced Glutathione ligand is immobilised to the carefully designed magnetic support via very stable thiolether linkages. 

GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single purification step. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet. GST tag can be cleaved in a bound condition or in an eluted condition by specific proteases.

Product Properties:

SepFast MAG is specially designed and fabricated for magnetic purification of proteins in batch mode (i.e. stirred tank mode). The base matrix is made of cross-linked agarose containing magnetic material. Therefore, the resin possesses magnetic properties. Removal of liquid after each step such as binding, washing and elution can be readily done by fixing the resin with a magnet.

Its carefully controlled pore structure allows fast accessto affinity ligands by target protein molecules. Also, its high mechanical strength permits harsher operational conditions. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) could be directly processed with Glutathione SepFast MAG. All these translate into high process flexibility and higher protein yield at shortened purification time.