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MISSION siRNA 轉染試劑
- 品牌：merck
- 產(chǎn)地：美國
- 型號：100 μL
- 貨號：S1452-100UL
- 發(fā)布日期： 2022-09-20
- 更新日期： 2024-08-12
產(chǎn)品詳請
| 產(chǎn)地 | 美國 |
| 品牌 | merck |
| 貨號 | S1452-100UL |
| 用途 | 細胞轉染 |
| 包裝規(guī)格 | 100 μL |
| 純度 | 詳詢% |
| CAS編號 | |
| 是否進口 | 否 |
General description:The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Other Notes:Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Other Notes:Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.
