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大腸桿菌表達(dá)質(zhì)粒 V012683
- 英文名稱：pTrc99a vector (V012683#)
- 品牌：novoprolabs
- 產(chǎn)地：國(guó)外
- 型號(hào)：4176 bp
- 貨號(hào)：V012683
- 發(fā)布日期： 2022-08-29
- 更新日期： 2024-08-12
產(chǎn)品詳請(qǐng)
| 產(chǎn)地 | 國(guó)外 |
| 品牌 | novoprolabs |
| 貨號(hào) | V012683 |
| 用途 | 詳詢 |
| 英文名稱 | pTrc99a vector (V012683#) |
| 包裝規(guī)格 | 4176 bp |
| 純度 | 詳詢% |
| CAS編號(hào) | |
| 別名 | 詳詢 |
| 分子式 | 詳詢 |
| 是否進(jìn)口 | 是 |
Basic Vector Information
Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
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| Vector Name | pTrc99a | Antibiotic Resistance | Ampicillin |
| Length | 4176 bp | Type | Bacterial expression vector |
| Copy Number | High copy number | Promoter | trc |
| Cloning Method | Enzyme Cut | 5' Primer | GAGCGGATAACAATTTCACACAGG |
| 3' Primer | GATTTAATCTGTATCAGG | Expression Method | IPTG induced |
pTrc99a vector Vector Map
Plasmid Resuspension protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
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