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- 品牌：Zymo Research
- 產(chǎn)地：美國
- 型號(hào)：10 Preps
- 貨號(hào)：P2001
- 發(fā)布日期： 2022-08-25
- 更新日期： 2024-08-12
| 產(chǎn)地 | 美國 |
| 品牌 | Zymo Research |
| 貨號(hào) | P2001 |
| 用途 | 詳詢 |
| 包裝規(guī)格 | 10 Preps |
| 純度 | 詳詢% |
| CAS編號(hào) | |
| 是否進(jìn)口 | 是 |
Product Introduction：The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 μl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
Highlights：
● Fast: Purify His-tagged proteins from cell lysates in less than 5 minutes.
● Simple: Prepare pure protein for small-scale studies using a spin-column.
● Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
Affinity Matrix：Nickel charged agarose
Binding Capacity：1 mg
Elution Method：Imidazole gradient
Elution Volume：100 - 200 μl (150 μl is optimal)
Principle of Technology：Based on a nickel-charged His-Affinity Gel (IMAC).
Processing Time：5 minutes
Product Storage：Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.
Protein Purity：Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
Required Equipment：Microcentrifuge
Sample Type：Cell lysates or other complex protein mixtures containing His-tagged proteins.
